8 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Charleston | 1 | 4,366 | 4,609 | -0.91% | 910 | 5 | City |
| East Prairie | 2 | 2,819 | 2,938 | -0.7% | 2,255 | 1 | City |
| Miner | 3 | 885 | 909 | -0.45% | 192 | 5 | City |
| Bertrand | 4 | 697 | 717 | -0.43% | 846 | 1 | City |
| Wyatt | 5 | 203 | 215 | -0.98% | 173 | 1 | City |
| Anniston | 6 | 177 | 183 | -0.56% | 453 | 0 | Town |
| Diehlstadt | 7 | 156 | 159 | 0% | 2,108 | 0 | Village |
| Wilson City | 8 | 74 | 77 | 0% | 987 | 0 | Village |
Charleston is the largest city in Mississippi County, with a population of 4,366.
Wilson City is the smallest city in Mississippi County, with a population of 74.
There are 8 cities in Mississippi County, Missouri.