Charleston
- County:
- Mississippi County
- County Seat:
- Yes
- Area (mi²):
- 4.796
- State:
- Missouri
Charleston is a city located in Mississippi County, Missouri. Charleston has a 2026 population of 4,447. It is also the county seat of Mississippi County. Charleston is currently declining at a rate of -0.96% annually and its population has decreased by -3.51% since the most recent census, which recorded a population of 4,609 in 2020.