| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Piedmont | 1 | 1,855 | 1,900 | -0.38% | 870 | 2 | City |
| Greenville | 2 | 438 | 448 | -0.45% | 649 | 1 | City |
| Williamsville | 3 | 273 | 276 | -0.36% | 845 | 0 | City |
| Mill Spring | 4 | 159 | 159 | 0% | 361 | 0 | Village |
Piedmont is the largest city in Wayne County, with a population of 1,855.
Mill Spring is the smallest city in Wayne County, with a population of 159.
There are 4 cities in Wayne County, Missouri.