14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Sikeston | 1 | 15,967 | 16,262 | -0.37% | 931 | 17 | City |
| Scott City | 2 | 4,352 | 4,342 | 0.05% | 899 | 5 | City |
| Chaffee | 3 | 3,006 | 3,053 | -0.3% | 1,675 | 2 | City |
| Oran | 4 | 1,208 | 1,200 | 0.17% | 1,147 | 1 | City |
| Benton | 5 | 935 | 867 | 1.52% | 1,470 | 1 | City |
| Morley | 6 | 699 | 633 | 1.9% | 873 | 1 | City |
| Kelso | 7 | 548 | 555 | -0.18% | 1,729 | 0 | Village |
| Benton | 8 | 498 | 478 | 1.43% | 12 | 42 | Township |
| Benton | 9 | 430 | 441 | -0.69% | 14 | 31 | Township |
| New Hamburg | 10 | 231 | 416 | 6.94% | 260 | 1 | CDP |
| Perkins | 11 | 225 | 301 | 7.14% | 1,364 | 0 | CDP |
| Blodgett | 12 | 204 | 209 | -0.49% | 1,252 | 0 | Village |
| Vanduser | 13 | 189 | 179 | 1.07% | 1,477 | 0 | Village |
| Commerce | 14 | 40 | 42 | 0% | 116 | 0 | Village |
Sikeston is the largest city in Scott County, with a population of 15,967.
Commerce is the smallest city in Scott County, with a population of 40.
There are 14 cities in Scott County, Missouri.