8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| O'Neill | 1 | 3,577 | 3,571 | 0.03% | 1,529 | 2.34 | City |
| Atkinson | 2 | 1,312 | 1,306 | 0.08% | 802 | 1.64 | City |
| Stuart | 3 | 512 | 486 | 0.99% | 375 | 1.37 | Village |
| Ewing | 4 | 374 | 379 | -0.27% | 762 | 0.49 | Village |
| Chambers | 5 | 285 | 287 | 0% | 280 | 1.02 | Village |
| Page | 6 | 166 | 166 | 0% | 680 | 0.24 | Village |
| Inman | 7 | 98 | 100 | 0% | 343 | 0.29 | Village |
| Emmet | 8 | 46 | 46 | 0% | 178 | 0.26 | Village |
O'Neill is the largest city in Holt County, with a population of 3,577.
Emmet is the smallest city in Holt County.
There are 8 cities in Holt County, Nebraska.