45 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| O'Neill | 1 | 3,579 | 3,571 | 0.03% | 1,530 | 2 | City |
| Atkinson | 2 | 1,743 | 1,739 | -0.11% | 22 | 81 | Township |
| Atkinson | 3 | 1,317 | 1,306 | 0.15% | 805 | 2 | City |
| Grattan | 4 | 825 | 834 | -0.36% | 7 | 117 | Township |
| Stuart | 5 | 810 | 799 | -0.37% | 6 | 135 | Township |
| Stuart | 6 | 509 | 488 | 0.79% | 372 | 1 | Village |
| Chambers | 7 | 422 | 424 | -0.47% | 6 | 72 | Township |
| Ewing | 8 | 416 | 415 | 0.24% | 8 | 54 | Township |
| Ewing | 9 | 379 | 379 | 0% | 772 | 0 | Village |
| Verdigris | 10 | 311 | 315 | -0.64% | 6 | 54 | Township |
| Chambers | 11 | 288 | 288 | 0% | 283 | 1 | Village |
| Inman | 12 | 259 | 262 | -0.77% | 3 | 95 | Township |
| Page | 13 | 167 | 167 | 0% | 684 | 0 | Village |
| Sheridan | 14 | 163 | 166 | -0.61% | 2 | 80 | Township |
| Emmet | 15 | 137 | 139 | -0.72% | 2 | 71 | Township |
| Deloit | 16 | 104 | 105 | -0.95% | 2 | 54 | Township |
| Golden | 17 | 103 | 103 | 0% | 2 | 54 | Township |
| Inman | 18 | 98 | 100 | -1.01% | 343 | 0 | Village |
| Shields | 19 | 90 | 90 | 0% | 1 | 72 | Township |
| Pleasant View | 20 | 82 | 82 | 0% | 2 | 36 | Township |
| Wyoming | 21 | 78 | 78 | 0% | 1 | 72 | Township |
| Sand Creek | 22 | 76 | 76 | 0% | 1 | 81 | Township |
| Paddock | 23 | 70 | 70 | 0% | 1 | 74 | Township |
| Green Valley | 24 | 69 | 69 | 0% | 1 | 70 | Township |
| Conley | 25 | 65 | 65 | 0% | 1 | 72 | Township |
| Lake | 26 | 56 | 56 | 0% | 1 | 54 | Township |
| Iowa | 27 | 47 | 47 | 0% | 1 | 36 | Township |
| McClure | 28 | 47 | 47 | 0% | 1 | 54 | Township |
| Emmet | 29 | 46 | 46 | 0% | 178 | 0 | Village |
| Rock Falls | 30 | 46 | 46 | 0% | 1 | 36 | Township |
| Saratoga | 31 | 43 | 43 | 0% | 1 | 53 | Township |
| Belle | 32 | 41 | 41 | 0% | 1 | 36 | Township |
| Coleman | 33 | 41 | 41 | 0% | 1 | 53 | Township |
| Swan | 34 | 40 | 40 | 0% | 0.00 | 107 | Township |
| Scott | 35 | 39 | 39 | 0% | 1 | 66 | Township |
| Shamrock | 36 | 39 | 39 | 0% | 1 | 54 | Township |
| Cleveland | 37 | 38 | 38 | 0% | 0.00 | 86 | Township |
| Willowdale | 38 | 38 | 38 | 0% | 1 | 72 | Township |
| Fairview | 39 | 36 | 36 | 0% | 1 | 54 | Township |
| Antelope | 40 | 33 | 33 | 0% | 1 | 36 | Township |
| Steel Creek | 41 | 27 | 27 | 0% | 0.00 | 65 | Township |
| Holt Creek | 42 | 26 | 26 | 0% | 0.00 | 53 | Township |
| Francis | 43 | 24 | 24 | 0% | 0.00 | 54 | Township |
| Dustin | 44 | 21 | 21 | 0% | 0.00 | 60 | Township |
| Josie | 45 | 21 | 21 | 0% | 1 | 36 | Township |
O'Neill is the largest city in Holt County, with a population of 3,579.
Josie is the smallest city in Holt County, with a population of 21.
There are 45 cities in Holt County, Nebraska.