| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| O'Neill | 1 | 3,564 | 3,569 | -0.08% | 1,524 | 2 | City |
| Atkinson | 2 | 1,317 | 1,311 | 0.08% | 805 | 2 | City |
| Stuart | 3 | 509 | 487 | 0.59% | 372 | 1 | Village |
| Ewing | 4 | 372 | 377 | -0.27% | 758 | 0 | Village |
| Chambers | 5 | 286 | 289 | -0.35% | 281 | 1 | Village |
| Page | 6 | 164 | 168 | -0.61% | 672 | 0 | Village |
| Inman | 7 | 98 | 100 | -1.01% | 343 | 0 | Village |
| Emmet | 8 | 45 | 46 | 0% | 174 | 0 | Village |
O'Neill is the largest city in Holt County, with a population of 3,564.
Emmet is the smallest city in Holt County, with a population of 45.
There are 8 cities in Holt County, Nebraska.