8 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| O'Neill | 1 | 3,581 | 3,571 | 0.06% | 1,531 | 2 | City |
| Atkinson | 2 | 1,319 | 1,306 | 0.15% | 806 | 2 | City |
| Stuart | 3 | 513 | 488 | 0.79% | 375 | 1 | Village |
| Ewing | 4 | 379 | 379 | 0% | 772 | 0 | Village |
| Chambers | 5 | 288 | 288 | 0% | 283 | 1 | Village |
| Page | 6 | 167 | 167 | 0% | 684 | 0 | Village |
| Inman | 7 | 98 | 100 | 0% | 343 | 0 | Village |
| Emmet | 8 | 46 | 46 | 0% | 178 | 0 | Village |
O'Neill is the largest city in Holt County, with a population of 3,581.
Emmet is the smallest city in Holt County, with a population of 46.
There are 8 cities in Holt County, Nebraska.