| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nebraska City | 1 | 7,578 | 7,216 | 0.88% | 1,524 | 5 | City |
| Syracuse | 2 | 2,020 | 1,943 | 0.7% | 1,582 | 1 | City |
| Palmyra | 3 | 575 | 523 | 1.77% | 1,691 | 0 | Village |
| Unadilla | 4 | 307 | 294 | 0.66% | 962 | 0 | Village |
| Talmage | 5 | 207 | 201 | 0.49% | 1,262 | 0 | Village |
| Douglas | 6 | 180 | 170 | 0.56% | 818 | 0 | Village |
| Dunbar | 7 | 170 | 164 | 0.59% | 739 | 0 | Village |
| Otoe | 8 | 169 | 166 | 0% | 1,063 | 0 | Village |
| Burr | 9 | 55 | 53 | 0% | 640 | 0 | Village |
| Lorton | 10 | 38 | 35 | 0% | 884 | 0 | Village |
Nebraska City is the largest city in Otoe County, with a population of 7,578.
Lorton is the smallest city in Otoe County, with a population of 38.
There are 10 cities in Otoe County, Nebraska.