10 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nebraska City | 1 | 7,682 | 7,213 | 1.03% | 1,545 | 5 | City |
| Syracuse | 2 | 2,050 | 1,945 | 0.89% | 1,605 | 1 | City |
| Palmyra | 3 | 591 | 524 | 1.9% | 1,738 | 0 | Village |
| Unadilla | 4 | 310 | 294 | 0.98% | 972 | 0 | Village |
| Talmage | 5 | 212 | 202 | 0.95% | 1,293 | 0 | Village |
| Douglas | 6 | 182 | 170 | 1.11% | 827 | 0 | Village |
| Dunbar | 7 | 175 | 165 | 1.16% | 761 | 0 | Village |
| Otoe | 8 | 173 | 167 | 0.58% | 1,088 | 0 | Village |
| Burr | 9 | 57 | 53 | 1.79% | 663 | 0 | Village |
| Lorton | 10 | 39 | 35 | 2.63% | 907 | 0 | Village |
Nebraska City is the largest city in Otoe County, with a population of 7,682.
Lorton is the smallest city in Otoe County, with a population of 39.
There are 10 cities in Otoe County, Nebraska.