35 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Creighton | 1 | 1,094 | 1,149 | -1% | 904 | 1.21 | City |
| Bloomfield | 2 | 929 | 984 | -1.17% | 1,138 | 0.82 | City |
| Center township | 3 | 795 | 744 | 0.89% | 23 | 33.94 | Township |
| Hill township | 4 | 773 | 759 | 0.65% | 12 | 66.91 | Township |
| Crofton | 5 | 761 | 755 | 0.13% | 1,189 | 0.64 | City |
| Verdigre township | 6 | 627 | 630 | 0.48% | 18 | 35.60 | Township |
| Wausa | 7 | 570 | 589 | -0.7% | 1,088 | 0.52 | Village |
| Verdigre | 8 | 534 | 549 | -0.56% | 974 | 0.55 | Village |
| Santee | 9 | 425 | 422 | 0.24% | 754 | 0.56 | Village |
| Niobrara township | 10 | 416 | 417 | 0.24% | 19 | 22.47 | Township |
| Niobrara | 11 | 360 | 367 | -0.28% | 495 | 0.73 | Village |
| Eastern township | 12 | 257 | 254 | 0.39% | 7 | 35.54 | Township |
| Creighton township | 13 | 196 | 202 | -1.01% | 6 | 34.85 | Township |
| Center township | 14 | 184 | 188 | -1.08% | 5 | 35.80 | Township |
| North Frankfort township | 15 | 167 | 167 | 0% | 52 | 3.23 | Township |
| Morton township | 16 | 161 | 164 | -0.62% | 5 | 35.06 | Township |
| Peoria township | 17 | 138 | 141 | -0.72% | 4 | 35.71 | Township |
| Dolphin township | 18 | 130 | 130 | 0% | 4 | 36.15 | Township |
| Addison township | 19 | 123 | 125 | -0.81% | 3 | 35.77 | Township |
| Columbia township | 20 | 121 | 117 | 1.68% | 3 | 36.05 | Township |
| Cleveland township | 21 | 113 | 115 | -0.88% | 3 | 36.05 | Township |
| Dowling township | 22 | 109 | 107 | 0.93% | 3 | 36.12 | Township |
| Jefferson township | 23 | 106 | 108 | -0.93% | 3 | 35.61 | Township |
| Frankfort township | 24 | 94 | 89 | 2.17% | 5 | 18.23 | Township |
| Herrick township | 25 | 90 | 91 | 0% | 5 | 18.86 | Township |
| Central township | 26 | 82 | 80 | 1.23% | 2 | 35.94 | Township |
| Walnut Grove township | 27 | 80 | 80 | 0% | 1 | 53.34 | Township |
| Center | 28 | 72 | 74 | 0% | 686 | 0.10 | Village |
| Sparta township | 29 | 68 | 67 | 0% | 2 | 34.97 | Township |
| Spade township | 30 | 63 | 64 | 0% | 2 | 35.49 | Township |
| Logan township | 31 | 60 | 61 | 0% | 2 | 35.74 | Township |
| Winnetoon | 32 | 49 | 51 | 0% | 169 | 0.29 | Village |
| Bohemia township | 33 | 41 | 42 | 0% | 1 | 33.58 | Township |
| Verdel | 34 | 38 | 40 | 0% | 220 | 0.17 | Village |
| Lindy | 35 | 21 | 19 | 5% | 21 | 1 | CDP |
Creighton is the largest city in Knox County, with a population of 1,094.
Lindy is the smallest city in Knox County.
There are 35 cities in Knox County, Nebraska.