11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nebraska City | 1 | 7,604 | 7,213 | 1.04% | 1,529 | 5 | City |
| Syracuse | 2 | 2,032 | 1,945 | 0.84% | 1,591 | 1 | City |
| Palmyra | 3 | 580 | 524 | 1.93% | 1,706 | 0 | Village |
| Unadilla | 4 | 307 | 294 | 0.66% | 962 | 0 | Village |
| Douglas | 5 | 216 | 210 | -1.37% | 7 | 32 | Township |
| Talmage | 6 | 210 | 202 | 0.48% | 1,280 | 0 | Village |
| Douglas | 7 | 180 | 170 | 1.12% | 818 | 0 | Village |
| Dunbar | 8 | 173 | 165 | 0.58% | 752 | 0 | Village |
| Otoe | 9 | 172 | 167 | 0.58% | 1,082 | 0 | Village |
| Burr | 10 | 56 | 53 | 0% | 651 | 0 | Village |
| Lorton | 11 | 38 | 35 | 0% | 884 | 0 | Village |
Nebraska City is the largest city in Otoe County, with a population of 7,604.
Lorton is the smallest city in Otoe County, with a population of 38.
There are 11 cities in Otoe County, Nebraska.