11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nebraska City | 1 | 7,548 | 7,212 | 0.9% | 1,518 | 4.97 | City |
| Syracuse | 2 | 1,998 | 1,938 | 0.6% | 1,565 | 1.28 | City |
| Palmyra | 3 | 591 | 531 | 2.07% | 1,738 | 0.34 | Village |
| Unadilla | 4 | 305 | 294 | 0.66% | 956 | 0.32 | Village |
| Douglas township | 5 | 225 | 209 | 0.9% | 7 | 31.98 | Township |
| Talmage | 6 | 207 | 201 | 0.49% | 1,262 | 0.16 | Village |
| Douglas | 7 | 174 | 171 | 0.58% | 791 | 0.22 | Village |
| Dunbar | 8 | 170 | 164 | 0.59% | 739 | 0.23 | Village |
| Otoe | 9 | 168 | 162 | 0.6% | 1,057 | 0.16 | Village |
| Burr | 10 | 54 | 53 | 0% | 628 | 0.09 | Village |
| Lorton | 11 | 35 | 34 | 0% | 814 | 0.04 | Village |
Nebraska City is the largest city in Otoe County, with a population of 7,548.
Lorton is the smallest city in Otoe County.
There are 11 cities in Otoe County, Nebraska.