12 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Helena | 1 | 1,533 | 1,533 | 0% | 3,757 | 0 | Town |
| Cherokee | 2 | 1,449 | 1,466 | -0.28% | 710 | 2 | City |
| Carmen | 3 | 359 | 359 | 0% | 236 | 2 | Town |
| Goltry | 4 | 252 | 251 | 0% | 630 | 0 | Town |
| Jet | 5 | 196 | 198 | -0.51% | 721 | 0 | Town |
| Aline | 6 | 171 | 170 | 0% | 645 | 0 | Town |
| Nescatunga | 7 | 161 | 69 | 7.33% | 712 | 0 | CDP |
| Burlington | 8 | 121 | 120 | 0% | 443 | 0 | Town |
| Byron | 9 | 41 | 41 | 0% | 163 | 0 | Town |
| Amorita | 10 | 40 | 40 | 0% | 158 | 0 | Town |
| Avard | 11 | 16 | 38 | 6.67% | 152 | 0 | CDP |
| Lambert | 12 | 6 | 6 | 0% | 54 | 0 | Town |
Helena is the largest city in Alfalfa County, with a population of 1,533.
Lambert is the smallest city in Alfalfa County, with a population of 6.
There are 12 cities in Alfalfa County, Oklahoma.