14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Hilton Head Island | 1 | 38,301 | 37,586 | 0.37% | 926 | 41 | Town |
| Bluffton | 2 | 38,101 | 28,324 | 5.41% | 731 | 52 | Town |
| Port Royal | 3 | 16,988 | 14,581 | 2.91% | 1,001 | 17 | Town |
| Beaufort | 4 | 14,511 | 13,625 | 1.23% | 594 | 24 | City |
| Burton | 5 | 7,760 | 8,779 | 0.67% | 929 | 8 | CDP |
| Laurel Bay | 6 | 5,418 | 6,388 | -0.59% | 1,301 | 4 | CDP |
| Shell Point | 7 | 1,949 | 2,991 | -1.27% | 486 | 4 | CDP |
| Seabrook | 8 | 1,359 | 1,456 | 7.18% | 247 | 6 | CDP |
| Dale | 9 | 1,196 | 699 | 7.17% | 504 | 2 | CDP |
| Fripp Island | 10 | 959 | 848 | 7.15% | 294 | 3 | CDP |
| Daufuskie Island | 11 | 711 | 485 | 7.08% | 72 | 10 | CDP |
| Sheldon | 12 | 549 | 501 | 7.23% | 126 | 4 | CDP |
| Harbor Island | 13 | 346 | 316 | 7.12% | 337 | 1 | CDP |
| Lobeco | 14 | 245 | 349 | 6.99% | 93 | 3 | CDP |
Hilton Head Island is the largest city in Beaufort County, with a population of 38,301.
Lobeco is the smallest city in Beaufort County, with a population of 245.
There are 14 cities in Beaufort County, South Carolina.