7 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Walterboro | 1 | 5,454 | 5,506 | -0.18% | 797 | 7 | City |
| Cottageville | 2 | 717 | 701 | 0.42% | 210 | 3 | Town |
| Jacksonboro | 3 | 218 | 200 | -7.23% | 15 | 14 | CDP |
| Smoaks | 4 | 97 | 99 | 0% | 60 | 2 | Town |
| Williams | 5 | 95 | 97 | 0% | 112 | 1 | Town |
| Lodge | 6 | 81 | 83 | 0% | 26 | 3 | Town |
| Islandton | 7 | 50 | 49 | 6.38% | 18 | 3 | CDP |
Walterboro is the largest city in Colleton County, with a population of 5,454.
Islandton is the smallest city in Colleton County, with a population of 50.
There are 7 cities in Colleton County, South Carolina.