9 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Florence | 1 | 41,336 | 40,096 | 0.5% | 1,659 | 25 | City |
| Lake City | 2 | 5,859 | 6,030 | -0.48% | 1,075 | 5 | City |
| Timmonsville | 3 | 2,126 | 2,098 | 0.24% | 766 | 3 | Town |
| Johnsonville | 4 | 1,347 | 1,356 | -0.07% | 637 | 2 | City |
| Pamplico | 5 | 1,061 | 1,060 | 0% | 655 | 2 | Town |
| Coward | 6 | 717 | 710 | 0.14% | 222 | 3 | Town |
| Scranton | 7 | 657 | 653 | 0.15% | 830 | 1 | Town |
| Olanta | 8 | 548 | 553 | -0.18% | 551 | 1 | Town |
| Stuckey | 9 | 185 | 196 | -1.07% | 198 | 1 | Town |
Florence is the largest city in Florence County, with a population of 41,336.
Stuckey is the smallest city in Florence County, with a population of 185.
There are 9 cities in Florence County, South Carolina.