| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Florence | 1 | 41,470 | 39,969 | 0.58% | 1,664 | 25 | City |
| Lake City | 2 | 5,916 | 6,025 | -0.4% | 1,086 | 5 | City |
| Timmonsville | 3 | 2,123 | 2,094 | 0.28% | 764 | 3 | Town |
| Johnsonville | 4 | 1,347 | 1,358 | -0.07% | 637 | 2 | City |
| Pamplico | 5 | 1,063 | 1,060 | 0.09% | 656 | 2 | Town |
| Coward | 6 | 730 | 724 | 0.14% | 226 | 3 | Town |
| Scranton | 7 | 653 | 655 | 0% | 824 | 1 | Town |
| Olanta | 8 | 549 | 554 | -0.18% | 552 | 1 | Town |
| Stuckey | 9 | 184 | 194 | -1.08% | 197 | 1 | Town |
Florence is the largest city in Florence County, with a population of 41,470.
Stuckey is the smallest city in Florence County, with a population of 184.
There are 9 cities in Florence County, South Carolina.