9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Madison | 1 | 2,674 | 2,897 | -1.66% | 382 | 7 | City |
| Danville | 2 | 627 | 671 | -1.42% | 586 | 1 | Town |
| Racine | 3 | 507 | 476 | 3.47% | 1,058 | 0 | CDP |
| Whitesville | 4 | 335 | 368 | -2.05% | 1,091 | 0 | Town |
| Greenview | 5 | 204 | 281 | -5.56% | 207 | 1 | CDP |
| Sylvester | 6 | 148 | 163 | -1.99% | 587 | 0 | Town |
| Comfort | 7 | 143 | 225 | -7.14% | 142 | 1 | CDP |
| Van | 8 | 114 | 138 | -5% | 191 | 1 | CDP |
| Twilight | 9 | 104 | 42 | 7.22% | 208 | 1 | CDP |
Madison is the largest city in Boone County, with a population of 2,674.
Twilight is the smallest city in Boone County, with a population of 104.
There are 9 cities in Boone County, West Virginia.