8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Wheeling | 1 | 25,846 | 26,986 | -0.82% | 1,875 | 14 | City |
| Wheeling | 2 | 25,846 | 26,913 | -0.82% | 1,875 | 14 | City |
| West Liberty | 3 | 1,430 | 1,452 | -0.28% | 1,310 | 1 | Town |
| West Liberty | 4 | 1,430 | 1,450 | -0.28% | 1,310 | 1 | Town |
| Triadelphia | 5 | 656 | 685 | -0.91% | 975 | 1 | Town |
| Triadelphia | 6 | 656 | 684 | -0.91% | 975 | 1 | Town |
| Valley Grove | 7 | 268 | 281 | -1.11% | 490 | 1 | Village |
| Valley Grove | 8 | 268 | 281 | -1.11% | 490 | 1 | Village |
Wheeling is the largest city in Ohio County, with a population of 25,846.
Valley Grove is the smallest city in Ohio County, with a population of 268.
There are 8 cities in Ohio County, West Virginia.