6 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Sistersville | 1 | 1,286 | 1,405 | -1.76% | 2,450 | 1 | City |
| Sistersville | 2 | 1,286 | 1,401 | -1.76% | 2,450 | 1 | City |
| Middlebourne | 3 | 660 | 715 | -1.64% | 1,828 | 0 | Town |
| Middlebourne | 4 | 660 | 714 | -1.64% | 1,828 | 0 | Town |
| Friendly | 5 | 92 | 99 | -2.13% | 911 | 0 | Town |
| Friendly | 6 | 92 | 99 | -2.13% | 911 | 0 | Town |
Sistersville is the largest city in Tyler County, with a population of 1,286.
Friendly is the smallest city in Tyler County, with a population of 92.
There are 6 cities in Tyler County, West Virginia.