3 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Sistersville | 1 | 1,263 | 1,401 | -1.79% | 2,406 | 1 | City |
| Middlebourne | 2 | 649 | 714 | -1.67% | 1,798 | 0 | Town |
| Friendly | 3 | 91 | 99 | -1.09% | 901 | 0 | Town |
Sistersville is the largest city in Tyler County, with a population of 1,263.
Friendly is the smallest city in Tyler County, with a population of 91.
There are 3 cities in Tyler County, West Virginia.