| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Sistersville | 1 | 1,299 | 1,403 | -1.52% | 2,474 | 1 | City |
| Middlebourne | 2 | 666 | 715 | -1.48% | 1,845 | 0 | Town |
| Friendly | 3 | 92 | 99 | -2.13% | 911 | 0 | Town |
Sistersville is the largest city in Tyler County, with a population of 1,299.
Friendly is the smallest city in Tyler County, with a population of 92.
There are 3 cities in Tyler County, West Virginia.