11 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mauston | 1 | 4,044 | 4,352 | -1.25% | 905 | 4 | City |
| New Lisbon | 2 | 2,590 | 2,577 | 0.08% | 948 | 3 | City |
| Elroy | 3 | 1,222 | 1,356 | -1.77% | 609 | 2 | City |
| Necedah | 4 | 903 | 936 | -0.55% | 327 | 3 | Village |
| Wonewoc | 5 | 710 | 761 | -1.11% | 636 | 1 | Village |
| Camp Douglas | 6 | 618 | 648 | -0.8% | 615 | 1 | Village |
| Lyndon Station | 7 | 467 | 496 | -1.06% | 221 | 2 | Village |
| Ironton | 8 | 255 | 273 | -1.16% | 766 | 0 | Village |
| Union Center | 9 | 215 | 227 | -0.92% | 261 | 1 | Village |
| Hustler | 10 | 160 | 169 | -0.62% | 229 | 1 | Village |
| Yuba | 11 | 50 | 52 | 0% | 164 | 0 | Village |
Mauston is the largest city in Juneau County, with a population of 4,044.
Yuba is the smallest city in Juneau County, with a population of 50.
There are 11 cities in Juneau County, Wisconsin.