24 cities
| City | County | 2026 Pop. ↓ |
|---|---|---|
| Omaha | Douglas County | 487,506 |
| Offutt AFB | Sarpy County | 5,898 |
| Ogallala | Keith County | 4,691 |
| O'Neill | Holt County | 3,581 |
| Ord | Valley County | 2,080 |
| Oakland | Burt County | 1,346 |
| Osceola | Polk County | 892 |
| Osmond | Pierce County | 803 |
| Oshkosh | Garden County | 744 |
| Oxford | Furnas County | 681 |
| Overton | Dawson County | 588 |
| Orchard | Antelope County | 347 |
| Orleans | Harlan County | 320 |
| Oakdale | Antelope County | 270 |
| Odell | Gage County | 254 |
| Overland | Hamilton County | 226 |
| Otoe | Otoe County | 173 |
| Oconto | Custer County | 141 |
| Ohiowa | Fillmore County | 121 |
| Odessa | Buffalo County | 113 |
| Octavia | Butler County | 108 |
| Oak | Nuckolls County | 52 |
| Ong | Clay County | 51 |
| Obert | Cedar County | 20 |