City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Binghamton | 1 | Broome County | 46,372 |
| Johnson City | 2 | Broome County | 14,745 |
| Endicott | 3 | Broome County | 13,069 |
| Sidney | 4 | Delaware County | 3,644 |
| Port Dickinson | 5 | Broome County | 1,652 |
| Greene | 6 | Chenango County | 1,413 |
| Deposit | 7 | Broome County | 1,410 |
| Oxford | 8 | Chenango County | 1,325 |
| Bainbridge | 9 | Chenango County | 1,247 |
| Unadilla | 10 | Otsego County | 1,083 |
| Otego | 11 | Otsego County | 906 |
| Hancock | 12 | Delaware County | 900 |
| Whitney Point | 13 | Broome County | 892 |
| Marathon | 14 | Cortland County | 873 |
| Windsor | 15 | Broome County | 855 |
| Afton | 16 | Chenango County | 781 |
| Lisle | 17 | Broome County | 322 |
| Franklin | 18 | Delaware County | 276 |