| City | 2025 Pop. ↓ | Growth |
|---|---|---|
| Prague | 1,331,940 | 0.3% |
| Brno | 380,587 | 0.14% |
| Ostrava | 283,361 | 1% |
| Liberec | 105,998 | 0.98% |
| Olomouc | 101,541 | 0.99% |
| Ceske Budejovice | 96,513 | 0.99% |
| Hradec Kralove | 93,002 | 0.99% |
| Pardubice | 90,488 | 0.98% |
| Usti nad Labem | 89,581 | 0.98% |
| Zlin | 74,042 | 1% |
| Most | 61,097 | 0.96% |
| Opava | 54,397 | 0.98% |
| Jihlava | 51,581 | 0.96% |
| Teplice | 48,614 | 0.95% |
| Karvina | 47,121 | 0.95% |
| Karlovy Vary | 46,326 | 0.94% |
| Decin | 45,868 | 0.98% |
| Chomutov | 45,137 | 0.96% |
| Jablonec nad Nisou | 45,122 | 0.98% |
| Mlada Boleslav | 43,416 | 0.94% |
| Prostejov | 43,387 | 1% |
| Prerov | 40,656 | 0.98% |
| Trinec | 33,512 | 0.98% |
