| City | 2026 Pop. ↓ | Growth |
|---|---|---|
| Prague | 1,335,350 | 0.26% |
| Brno | 381,161 | 0.15% |
| Ostrava | 282,300 | 1% |
| Liberec | 105,997 | 1% |
| Olomouc | 101,552 | 1% |
| Ceske Budejovice | 96,617 | 1% |
| Hradec Kralove | 92,697 | 1% |
| Pardubice | 90,186 | 1% |
| Usti nad Labem | 88,974 | 0.99% |
| Zlin | 73,864 | 1% |
| Kladno | 67,721 | 0.99% |
| Most | 59,996 | 0.98% |
| Opava | 53,900 | 0.99% |
| Jihlava | 51,015 | 0.99% |
| Teplice | 48,206 | 0.99% |
| Karvina | 46,174 | 0.98% |
| Decin | 45,590 | 0.99% |
| Karlovy Vary | 45,360 | 0.98% |
| Jablonec nad Nisou | 44,755 | 0.99% |
| Chomutov | 44,591 | 0.99% |
| Prostejov | 43,253 | 1% |
| Mlada Boleslav | 42,848 | 0.99% |
| Prerov | 40,150 | 0.99% |
