30 cities
| City | County | 2026 Pop. ↓ |
|---|---|---|
| Alafaya | Orange County | 93,819 |
| Apopka | Orange County | 66,701 |
| Altamonte Springs | Seminole County | 47,805 |
| Aventura | Miami-Dade County | 40,775 |
| Apollo Beach | Hillsborough County | 31,645 |
| Auburndale | Polk County | 23,117 |
| Azalea Park | Orange County | 16,930 |
| Asbury Lake | Clay County | 14,565 |
| Atlantic Beach | Duval County | 13,172 |
| Alachua | Alachua County | 10,994 |
| Avon Park | Highlands County | 10,791 |
| Ave Maria | Collier County | 8,401 |
| Arcadia | DeSoto County | 8,240 |
| Alturas | Polk County | 3,792 |
| Alva | Lee County | 3,437 |
| Apalachicola | Franklin County | 2,522 |
| Astatula | Lake County | 2,449 |
| Atlantis | Palm Beach County | 2,179 |
| Astor | Lake County | 1,496 |
| Allentown | Santa Rosa County | 1,271 |
| Archer | Alachua County | 1,192 |
| Avalon | Santa Rosa County | 1,115 |
| Anna Maria | Manatee County | 1,039 |
| Andrews | Levy County | 723 |
| Alford | Jackson County | 532 |
| Altha | Calhoun County | 497 |
| Acacia Villas | Palm Beach County | 257 |
| Aucilla | Jefferson County | 191 |
| Aripeka | Pasco County | 163 |
| Altoona | Lake County | 41 |