23 cities
| City | County | 2026 Pop. ↓ |
|---|---|---|
| Estero | Lee County | 39,137 |
| Egypt Lake-Leto | Hillsborough County | 35,808 |
| East Lake | Pinellas County | 33,494 |
| East Lake-Orient Park | Hillsborough County | 30,520 |
| Eustis | Lake County | 25,984 |
| Edgewater | Volusia County | 24,928 |
| Ensley | Escambia County | 23,598 |
| Englewood | Charlotte County | 20,429 |
| East Milton | Santa Rosa County | 15,020 |
| Elfers | Pasco County | 13,852 |
| Eagle Lake | Polk County | 6,836 |
| Ellenton | Manatee County | 5,726 |
| Eglin AFB | Okaloosa County | 3,137 |
| Edgewood | Orange County | 2,954 |
| Eastpoint | Franklin County | 2,663 |
| East Bronson | Alachua County | 2,386 |
| Eatonville | Orange County | 2,367 |
| El Portal | Miami-Dade County | 1,982 |
| East Palatka | Putnam County | 1,509 |
| East Williston | Levy County | 666 |
| Everglades | Collier County | 415 |
| Esto | Holmes County | 355 |
| Ebro | Washington County | 251 |