23 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Estero | Lee County | 38,790 |
| Egypt Lake-Leto | Hillsborough County | 35,775 |
| East Lake | Pinellas County | 33,335 |
| East Lake-Orient Park | Hillsborough County | 30,035 |
| Eustis | Lake County | 25,525 |
| Edgewater | Volusia County | 24,631 |
| Ensley | Escambia County | 23,410 |
| Englewood | Charlotte County | 20,081 |
| East Milton | Santa Rosa County | 14,773 |
| Elfers | Pasco County | 13,860 |
| Eagle Lake | Polk County | 6,206 |
| Ellenton | Manatee County | 5,635 |
| Eglin AFB | Okaloosa County | 3,083 |
| Edgewood | Orange County | 2,910 |
| Eastpoint | Franklin County | 2,643 |
| Eatonville | Orange County | 2,361 |
| East Bronson | Alachua County | 2,358 |
| El Portal | Miami-Dade County | 1,981 |
| East Palatka | Putnam County | 1,518 |
| East Williston | Levy County | 667 |
| Everglades | Collier County | 405 |
| Esto | Holmes County | 352 |
| Ebro | Washington County | 248 |