14 cities
| City | County | 2026 Pop. ↓ |
|---|---|---|
| Joplin | Jasper County | 54,502 |
| Jefferson City | Cole County | 42,582 |
| Jackson | Cape Girardeau County | 16,167 |
| Jennings | St. Louis County | 13,085 |
| Jasper | Jasper County | 865 |
| Jonesburg | Montgomery County | 859 |
| Jamesport | Daviess County | 570 |
| Josephville | St. Charles County | 536 |
| Jane | McDonald County | 422 |
| Jamestown | Moniteau County | 332 |
| Junction City | Madison County | 286 |
| Jerico Springs | Cedar County | 174 |
| Jacksonville | Randolph County | 120 |
| Jameson | Daviess County | 74 |