14 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Joplin | Jasper County | 54,053 |
| Jefferson City | Cole County | 42,573 |
| Jackson | Cape Girardeau County | 16,048 |
| Jennings | St. Louis County | 13,054 |
| Jasper | Jasper County | 871 |
| Jonesburg | Montgomery County | 836 |
| Jamesport | Daviess County | 568 |
| Josephville | St. Charles County | 526 |
| Jane | McDonald County | 411 |
| Jamestown | Moniteau County | 331 |
| Junction City | Madison County | 286 |
| Jerico Springs | Cedar County | 171 |
| Jacksonville | Randolph County | 120 |
| Jameson | Daviess County | 73 |