| City | County | 2026 Pop. ↓ |
|---|---|---|
| Joplin | Jasper County | 54,391 |
| Jefferson City | Cole County | 42,529 |
| Jackson | Cape Girardeau County | 16,143 |
| Jennings | St. Louis County | 13,120 |
| Jonesburg | Montgomery County | 887 |
| Jasper | Jasper County | 864 |
| Jamesport | Daviess County | 571 |
| Josephville | St. Charles County | 538 |
| Jane | McDonald County | 414 |
| Jamestown | Moniteau County | 332 |
| Junction City | Madison County | 287 |
| Jerico Springs | Cedar County | 170 |
| Jacksonville | Randolph County | 122 |
| Jameson | Daviess County | 71 |