| City | County | 2026 Pop. ↓ |
|---|---|---|
| Eldridge | Scott County | 7,005 |
| Estherville | Emmet County | 5,780 |
| Evansdale | Black Hawk County | 4,443 |
| Emmetsburg | Palo Alto County | 3,680 |
| Eagle Grove | Wright County | 3,664 |
| Eldora | Hardin County | 2,579 |
| Ely | Linn County | 2,368 |
| Epworth | Dubuque County | 2,039 |
| Earlham | Madison County | 1,394 |
| Elkader | Clayton County | 1,179 |
| Elkhart | Polk County | 1,143 |
| Elk Run Heights | Black Hawk County | 1,050 |
| Eddyville | Wapello County | 959 |
| Edgewood | Clayton County | 907 |
| Eldon | Wapello County | 775 |
| Exira | Audubon County | 762 |
| Essex | Page County | 710 |
| Earlville | Delaware County | 706 |
| Elgin | Fayette County | 667 |
| Elk Horn | Shelby County | 624 |
| Early | Sac County | 563 |
| Everly | Clay County | 560 |
| Ellsworth | Hamilton County | 494 |
| Elma | Howard County | 489 |
| Emerson | Mills County | 401 |
| Earling | Shelby County | 386 |
| Elliott | Montgomery County | 321 |
| Elberon | Tama County | 177 |
| Exline | Appanoose County | 153 |
| East Peru | Clarke County | 120 |
| East Amana | Iowa County | 65 |
| Elkport | Clayton County | 27 |
| Ellston | Ringgold County | 21 |