City | 2024 Population |
|---|---|
| Oak Hill | 2,164 |
| Oak Ridge | 25,356 |
| Oakland | 3,662 |
| Oakland Park | 44,092 |
| Oakleaf Plantation | 30,179 |
| Ocala | 69,965 |
| Ocala Estates | 2,631 |
| Ocean Breeze | 433 |
| Ocean City | 6,247 |
| Ocean Ridge | 1,845 |
| Ocklawaha | 1,770 |
| Ocoee | 49,841 |
| Odessa | 7,268 |
| Ojus | 16,290 |
| Okahumpka | 297 |
| Okeechobee | 5,719 |
| Old Miakka | 1,670 |
| Oldsmar | 14,801 |
| Olga | 2,733 |
| Olympia Heights | 14,082 |
| On Top World | 15,196 |
| Ona | 475 |
| Opa-locka | 15,597 |
| Orange City | 15,670 |
| Orange Park | 9,055 |
| Orangetree | 5,627 |
| Orchid | 557 |
| Oriole Beach | 1,715 |
| Orlando | 325,044 |
| Orlovista | 5,854 |
| Ormond Beach | 44,663 |
| Ormond-by-the-Sea | 6,337 |
| Osprey | 6,172 |
| Otter Creek | 113 |
| Oviedo | 41,637 |