City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Andalusia | 1 | Covington County | 8,838 |
| Greenville | 2 | Butler County | 6,919 |
| Monroeville | 3 | Monroe County | 5,666 |
| Brewton | 4 | Escambia County | 5,138 |
| Evergreen | 5 | Conecuh County | 3,309 |
| East Brewton | 6 | Conecuh County | 2,239 |
| Camden | 7 | Wilcox County | 1,664 |
| Flomaton | 8 | Escambia County | 1,448 |
| Georgiana | 9 | Butler County | 1,256 |
| Fort Deposit | 10 | Lowndes County | 1,128 |
| Mosses | 11 | Lowndes County | 774 |
| Hayneville | 12 | Lowndes County | 759 |
| River Falls | 13 | Covington County | 495 |
| McKenzie | 14 | Butler County | 488 |
| Castleberry | 15 | Conecuh County | 462 |
| Lockhart | 16 | Covington County | 447 |
| Red Level | 17 | Covington County | 435 |
| Boykin | 18 | Wilcox County | 310 |
| Carolina | 19 | Covington County | 291 |
| Dozier | 20 | Crenshaw County | 283 |
| Sanford | 21 | Covington County | 267 |
| Heath | 22 | Conecuh County | 243 |
| Gordonville | 23 | Lowndes County | 227 |
| Repton | 24 | Conecuh County | 226 |
| Vredenburgh | 25 | Monroe County | 217 |
| Horn Hill | 26 | Covington County | 209 |
| Gantt | 27 | Covington County | 203 |
| Beatrice | 28 | Monroe County | 198 |
| Riverview | 29 | Conecuh County | 164 |
| Pine Apple | 30 | Wilcox County | 126 |
| Pollard | 31 | Escambia County | 125 |
| Libertyville | 32 | Covington County | 108 |
| Peterman | 33 | Monroe County | 36 |
| Oak Hill | 34 | Wilcox County | 13 |