City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Alliance | 1 | Box Butte County | 8,023 |
| Chadron | 2 | Dawes County | 5,134 |
| Valentine | 3 | Cherry County | 2,632 |
| Gordon | 4 | Sheridan County | 1,426 |
| Crawford | 5 | Dawes County | 819 |
| Rushville | 6 | Sheridan County | 774 |
| Hemingford | 7 | Box Butte County | 761 |
| Hay Springs | 8 | Sheridan County | 567 |
| Mullen | 9 | Hooker County | 449 |
| Harrison | 10 | Sioux County | 240 |
| Cody | 11 | Cherry County | 167 |
| Merriman | 12 | Cherry County | 88 |
| Crookston | 13 | Cherry County | 72 |
| Kilgore | 14 | Cherry County | 68 |
| Whitney | 15 | Dawes County | 58 |
| Clinton | 16 | Cherry County | 37 |
| Berea | 17 | Box Butte County | 35 |
| Seneca | 18 | Thomas County | 35 |
| Nenzel | 19 | Cherry County | 17 |
| Brownlee | 20 | Cherry County | 8 |