9 cities
| City | Rank | County | 2025 Pop. ↓ |
|---|---|---|---|
| Martinsburg | 1 | Berkeley County | 19,042 |
| Charles Town | 2 | Jefferson County | 8,436 |
| Ranson | 3 | Jefferson County | 5,854 |
| Shepherdstown | 4 | Jefferson County | 1,541 |
| Bolivar | 5 | Jefferson County | 984 |
| Bath (Berkeley Springs) | 6 | Morgan County | 750 |
| Paw Paw | 7 | Morgan County | 408 |
| Hedgesville | 8 | Berkeley County | 299 |
| Harpers Ferry | 9 | Jefferson County | 266 |
Berkeley County, Jefferson County, and Morgan County are the counties generally considered part of Eastern Panhandle West Virginia.