City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Morgantown | 1 | Monongalia County | 30,509 |
| Martinsburg | 2 | Berkeley County | 18,988 |
| Charles Town | 3 | Jefferson County | 8,054 |
| Ranson | 4 | Jefferson County | 5,776 |
| Keyser | 5 | Mineral County | 4,838 |
| Grafton | 6 | Taylor County | 4,544 |
| Westover | 7 | Monongalia County | 4,067 |
| Kingwood | 8 | Preston County | 2,948 |
| Star City | 9 | Monongalia County | 1,784 |
| Romney | 10 | Hampshire County | 1,749 |
| Shepherdstown | 11 | Jefferson County | 1,539 |
| Terra Alta | 12 | Preston County | 1,410 |
| Granville | 13 | Monongalia County | 1,375 |
| Bolivar | 14 | Jefferson County | 994 |
| Carpendale | 15 | Mineral County | 843 |
| Bath (Berkeley Springs) | 16 | Morgan County | 751 |
| Piedmont | 17 | Mineral County | 697 |
| Ridgeley | 18 | Mineral County | 573 |
| Reedsville | 19 | Preston County | 524 |
| Masontown | 20 | Preston County | 504 |
| Rowlesburg | 21 | Preston County | 433 |
| Capon Bridge | 22 | Hampshire County | 424 |
| Paw Paw | 23 | Morgan County | 409 |
| Hedgesville | 24 | Berkeley County | 300 |
| Tunnelton | 25 | Preston County | 290 |
| Harpers Ferry | 26 | Jefferson County | 267 |
| Newburg | 27 | Preston County | 256 |
| Albright | 28 | Preston County | 245 |
| Elk Garden | 29 | Mineral County | 205 |
| Bayard | 30 | Grant County | 191 |
| Brandonville | 31 | Preston County | 127 |
| Bruceton Mills | 32 | Preston County | 61 |