City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Clarksville | 1 | Montgomery County | 185,118 |
| Springfield | 2 | Robertson County | 19,723 |
| Union City | 3 | Obion County | 10,885 |
| Pleasant View | 4 | Cheatham County | 5,692 |
| Coopertown | 5 | Cheatham County | 4,768 |
| Tiptonville | 6 | Lake County | 3,256 |
| South Fulton | 7 | Obion County | 2,183 |
| Dover | 8 | Stewart County | 1,888 |
| Puryear | 9 | Henry County | 710 |
| Adams | 10 | Robertson County | 663 |
| Woodland Mills | 11 | Obion County | 337 |
| Cumberland City | 12 | Stewart County | 318 |
| Cedar Hill | 13 | Robertson County | 298 |
| Big Rock | 14 | Stewart County | 292 |
| Samburg | 15 | Obion County | 198 |
| Palmersville | 16 | Weakley County | 166 |
| Dukedom | 17 | Weakley County | 128 |
| Buchanan | 18 | Henry County | 86 |
| Cottage Grove | 19 | Henry County | 66 |
| Whitlock | 20 | Henry County | 21 |