City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Burlington | 1 | Alamance County | 60,877 |
| Mebane | 2 | Alamance County | 20,872 |
| Eden | 3 | Rockingham County | 15,307 |
| Reidsville | 4 | Rockingham County | 14,583 |
| Elon | 5 | Alamance County | 11,583 |
| Hillsborough | 6 | Orange County | 9,788 |
| Gibsonville | 7 | Guilford County | 9,615 |
| Roxboro | 8 | Person County | 8,235 |
| Glen Raven | 9 | Alamance County | 3,870 |
| Green Level | 10 | Alamance County | 3,340 |
| Wentworth | 11 | Rockingham County | 2,733 |
| Haw River | 12 | Alamance County | 2,379 |
| Yanceyville | 13 | Caswell County | 2,029 |
| Rougemont | 14 | Durham County | 1,150 |
| Woodlawn | 15 | Alamance County | 955 |
| McLeansville | 16 | Alamance County | 918 |
| Efland | 17 | Orange County | 840 |
| Sedalia | 18 | Guilford County | 688 |
| Whitsett | 19 | Guilford County | 595 |
| Ossipee | 20 | Alamance County | 563 |
| Ruffin | 21 | Rockingham County | 448 |
| Altamahaw | 22 | Alamance County | 437 |
| Milton | 23 | Caswell County | 154 |