City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Tuscaloosa | 1 | Tuscaloosa County | 113,711 |
| Northport | 2 | Tuscaloosa County | 31,080 |
| Demopolis | 3 | Marengo County | 6,730 |
| Cottondale | 4 | Tuscaloosa County | 3,746 |
| Moundville | 5 | Hale County | 3,677 |
| Holt | 6 | Tuscaloosa County | 3,670 |
| Livingston | 7 | Sumter County | 3,092 |
| Eutaw | 8 | Greene County | 2,708 |
| York | 9 | Sumter County | 2,276 |
| Greensboro | 10 | Hale County | 2,147 |
| Aliceville | 11 | Pickens County | 2,018 |
| Coaling | 12 | Tuscaloosa County | 1,970 |
| Uniontown | 13 | Perry County | 1,919 |
| Linden | 14 | Marengo County | 1,822 |
| Carrollton | 15 | Pickens County | 965 |
| Coker | 16 | Tuscaloosa County | 873 |
| Pickensville | 17 | Greene County | 540 |
| Forkland | 18 | Greene County | 418 |
| Thomaston | 19 | Marengo County | 315 |
| Cuba | 20 | Sumter County | 278 |
| Boligee | 21 | Greene County | 275 |
| Epes | 22 | Sumter County | 259 |
| Akron | 23 | Hale County | 222 |
| Union | 24 | Greene County | 163 |
| Gainesville | 25 | Sumter County | 161 |
| Providence | 26 | Hale County | 160 |
| Geiger | 27 | Sumter County | 153 |
| Newbern | 28 | Hale County | 131 |
| Faunsdale | 29 | Marengo County | 83 |
| Panola | 30 | Sumter County | 76 |
| Bellamy | 31 | Sumter County | 41 |
| McMullen | 32 | Greene County | 28 |
| Dayton | 33 | Hale County | 26 |
| Memphis | 34 | Greene County | 24 |
| Emelle | 35 | Sumter County | 21 |