| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Windsor | 1 | 3,484 | 3,424 | 0.43% | 1,238 | 3 | Town |
| Aulander | 2 | 705 | 758 | -1.4% | 477 | 1 | Town |
| Lewiston Woodville | 3 | 399 | 430 | -1.48% | 204 | 2 | Town |
| Kelford | 4 | 202 | 212 | -0.98% | 419 | 0 | Town |
| Colerain | 5 | 195 | 212 | -1.52% | 741 | 0 | Town |
| Powellsville | 6 | 177 | 190 | -1.67% | 496 | 0 | Town |
| Roxobel | 7 | 174 | 183 | -1.14% | 167 | 1 | Town |
| Askewville | 8 | 169 | 181 | -1.17% | 285 | 1 | Town |
Windsor is the largest city in Bertie County, with a population of 3,484.
Askewville is the smallest city in Bertie County, with a population of 169.
There are 8 cities in Bertie County, North Carolina.