23 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Eugene | 1 | 178,213 | 177,428 | 0.09% | 4,009 | 44.45 | City |
| Springfield | 2 | 60,569 | 61,859 | -0.42% | 3,815 | 15.88 | City |
| Santa Clara | 3 | 14,221 | 11,020 | 7.14% | 3,043 | 4.67 | CDP |
| River Road | 4 | 10,844 | 10,352 | 7.14% | 5,988 | 1.81 | CDP |
| Cottage Grove | 5 | 10,629 | 10,613 | 0.03% | 2,746 | 3.87 | City |
| Florence | 6 | 9,465 | 9,477 | -0.02% | 1,708 | 5.54 | City |
| Junction City | 7 | 7,256 | 6,796 | 1.28% | 2,193 | 3.31 | City |
| Creswell | 8 | 5,566 | 5,643 | -0.27% | 3,245 | 1.72 | City |
| Veneta | 9 | 5,157 | 5,222 | -0.25% | 2,007 | 2.57 | City |
| Oakridge | 10 | 3,104 | 3,211 | -0.67% | 1,436 | 2.16 | City |
| Heceta Beach | 11 | 2,653 | 1,708 | 7.15% | 1,138 | 2.33 | CDP |
| Dunes City | 12 | 1,393 | 1,428 | -0.5% | 518 | 2.69 | City |
| Dexter | 13 | 1,335 | 815 | 7.14% | 726 | 1.84 | CDP |
| Coburg | 14 | 1,310 | 1,312 | 0% | 1,096 | 1.20 | City |
| Lowell | 15 | 1,290 | 1,204 | 1.33% | 1,491 | 0.86 | City |
| Jasper | 16 | 718 | 427 | 7.16% | 270 | 2.66 | CDP |
| San Marine | 17 | 660 | 532 | 7.14% | 586 | 1.13 | CDP |
| Mapleton | 18 | 561 | 332 | 7.06% | 624 | 0.90 | CDP |
| Elmira | 19 | 510 | 721 | 7.14% | 416 | 1.23 | CDP |
| Marcola | 20 | 500 | 509 | 7.07% | 412 | 1.22 | CDP |
| Trent | 21 | 406 | 260 | 7.12% | 256 | 1.58 | CDP |
| Cheshire | 22 | 333 | 167 | 7.42% | 709 | 0.47 | CDP |
| Westfir | 23 | 252 | 262 | -0.79% | 803 | 0.31 | City |
Eugene is the largest city in Lane County, with a population of 178,213.
Westfir is the smallest city in Lane County.
There are 23 cities in Lane County, Oregon.