23 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Eugene | 1 | 178,873 | 178,436 | 0.05% | 4,024 | 44 | City |
| Springfield | 2 | 61,011 | 61,865 | -0.28% | 3,843 | 16 | City |
| Santa Clara | 3 | 14,221 | 11,020 | 7.14% | 3,043 | 5 | CDP |
| River Road | 4 | 10,844 | 10,352 | 7.14% | 5,988 | 2 | CDP |
| Cottage Grove | 5 | 10,702 | 10,642 | 0.11% | 2,765 | 4 | City |
| Florence | 6 | 9,511 | 9,476 | 0.07% | 1,717 | 6 | City |
| Junction City | 7 | 7,389 | 6,721 | 1.83% | 2,234 | 3 | City |
| Creswell | 8 | 5,609 | 5,637 | -0.11% | 3,271 | 2 | City |
| Veneta | 9 | 5,186 | 5,226 | -0.15% | 2,018 | 3 | City |
| Oakridge | 10 | 3,145 | 3,210 | -0.41% | 1,455 | 2 | City |
| Heceta Beach | 11 | 2,653 | 1,708 | 7.15% | 1,138 | 2 | CDP |
| Dunes City | 12 | 1,410 | 1,430 | -0.28% | 525 | 3 | City |
| Coburg | 13 | 1,356 | 1,314 | 0.59% | 1,135 | 1 | City |
| Dexter | 14 | 1,335 | 815 | 7.14% | 726 | 2 | CDP |
| Lowell | 15 | 1,302 | 1,206 | 1.48% | 1,505 | 1 | City |
| Jasper | 16 | 718 | 427 | 7.16% | 270 | 3 | CDP |
| San Marine | 17 | 660 | 532 | 7.14% | 586 | 1 | CDP |
| Mapleton | 18 | 561 | 332 | 7.06% | 624 | 1 | CDP |
| Elmira | 19 | 510 | 721 | 7.14% | 416 | 1 | CDP |
| Marcola | 20 | 500 | 509 | 7.07% | 412 | 1 | CDP |
| Trent | 21 | 406 | 260 | 7.12% | 256 | 2 | CDP |
| Cheshire | 22 | 333 | 167 | 7.42% | 709 | 0 | CDP |
| Westfir | 23 | 253 | 260 | -0.78% | 806 | 0 | City |
Eugene is the largest city in Lane County, with a population of 178,873.
Westfir is the smallest city in Lane County, with a population of 253.
There are 23 cities in Lane County, Oregon.