9 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Holton | 1 | 3,432 | 3,401 | 0.15% | 1,283 | 3 | City |
| Hoyt | 2 | 597 | 594 | 0.17% | 1,273 | 0 | City |
| Mayetta | 3 | 363 | 350 | 0.55% | 1,973 | 0 | City |
| Whiting | 4 | 199 | 189 | 1.02% | 198 | 1 | City |
| Circleville | 5 | 154 | 154 | 0% | 609 | 0 | City |
| Delia | 6 | 154 | 150 | 0.65% | 1,339 | 0 | City |
| Denison | 7 | 148 | 145 | 0.68% | 1,321 | 0 | City |
| Netawaka | 8 | 141 | 141 | 0% | 146 | 1 | City |
| Soldier | 9 | 105 | 102 | 0.96% | 714 | 0 | City |
Holton is the largest city in Jackson County, with a population of 3,432.
Soldier is the smallest city in Jackson County, with a population of 105.
There are 9 cities in Jackson County, Kansas.