6 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Centreville | 1 | 4,783 | 4,720 | 0.25% | 1,748 | 3 | Town |
| Church Hill | 2 | 836 | 809 | 0.6% | 1,164 | 1 | Town |
| Queenstown | 3 | 745 | 705 | 1.09% | 371 | 2 | Town |
| Sudlersville | 4 | 517 | 509 | 0.19% | 340 | 2 | Town |
| Queen Anne | 5 | 191 | 194 | -0.52% | 1,384 | 0 | Town |
| Barclay | 6 | 180 | 184 | -0.55% | 455 | 0 | Town |
Centreville is the largest city in Queen Anne's County, with a population of 4,783.
Barclay is the smallest city in Queen Anne's County, with a population of 180.
There are 6 cities in Queen Anne's County, Maryland.