19 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Stevensville | 1 | 8,905 | 8,145 | 1.6% | 1,385 | 6 | CDP |
| Centreville | 2 | 4,783 | 4,727 | 0.25% | 1,748 | 3 | Town |
| Centreville | 3 | 4,783 | 4,720 | 0.25% | 1,748 | 3 | Town |
| Chester | 4 | 4,528 | 4,319 | 0.53% | 865 | 5 | CDP |
| Grasonville | 5 | 3,309 | 3,578 | -0.21% | 598 | 6 | CDP |
| Romancoke | 6 | 2,201 | 1,717 | 7.16% | 1,737 | 1 | CDP |
| Queen Anne | 7 | 1,575 | 1,183 | 1.29% | 181 | 9 | CDP |
| Church Hill | 8 | 836 | 809 | 0.6% | 1,164 | 1 | Town |
| Church Hill | 9 | 836 | 809 | 0.6% | 1,164 | 1 | Town |
| Queenstown | 10 | 745 | 705 | 1.09% | 371 | 2 | Town |
| Queenstown | 11 | 745 | 705 | 1.09% | 371 | 2 | Town |
| Kent Narrows | 12 | 581 | 506 | 0.17% | 617 | 1 | CDP |
| Sudlersville | 13 | 517 | 507 | 0.19% | 340 | 2 | Town |
| Sudlersville | 14 | 517 | 509 | 0.19% | 340 | 2 | Town |
| Crumpton | 15 | 450 | 324 | 7.14% | 479 | 1 | CDP |
| Queen Anne | 16 | 191 | 194 | -0.52% | 1,384 | 0 | Town |
| Queen Anne | 17 | 191 | 194 | -0.52% | 1,384 | 0 | Town |
| Barclay | 18 | 180 | 184 | -0.55% | 455 | 0 | Town |
| Barclay | 19 | 180 | 184 | -0.55% | 455 | 0 | Town |
Stevensville is the largest city in Queen Anne's County, with a population of 8,905.
Barclay is the smallest city in Queen Anne's County, with a population of 180.
There are 19 cities in Queen Anne's County, Maryland.