Note: As defined by the US Census Bureau, a city's official boundaries often extend miles beyond the city itself and may even cross county lines. As such, several thousand US cities officially exist in two or more counties (and therefore appear on multiple counties' lists of cities.)
City | Rank | 2024 Pop. | 2020 Pop. | Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Stevensville | 1 | 8,765 | 8,145 | 1.62% | 1,363 | 6.4 | CDP |
| Centreville | 2 | 4,752 | 4,722 | 0.15% | 1,736 | 2.7 | Town |
| Chester | 3 | 4,504 | 4,319 | 0.54% | 860 | 5.2 | CDP |
| Grasonville | 4 | 3,316 | 3,578 | -0.24% | 600 | 5.5 | CDP |
| Romancoke | 5 | 2,054 | 1,717 | 7.65% | 1,621 | 1.3 | CDP |
| Queen Anne | 6 | 1,555 | 1,183 | 1.24% | 178 | 8.7 | CDP |
| Church Hill | 7 | 822 | 808 | 0.37% | 1,145 | 0.7 | Town |
| Queenstown | 8 | 750 | 705 | 1.49% | 373 | 2.0 | Town |
| Kent Narrows | 9 | 580 | 506 | 0.17% | 616 | 0.9 | CDP |
| Sudlersville | 10 | 515 | 510 | 0.19% | 339 | 1.5 | Town |
| Crumpton | 11 | 420 | 324 | 7.69% | 447 | 0.9 | CDP |
| Queen Anne | 12 | 189 | 193 | -0.53% | 1,370 | 0.1 | Town |
| Barclay | 13 | 180 | 184 | -0.55% | 455 | 0.4 | Town |
Note: City markers shown outside the county boundary mean that a part of the city extends into this county, but is primarily located in an adjacent county.
Stevensville is the largest city in Queen Anne's County.
Barclay is the smallest city in Queen Anne's County.
There are 13 cities in Queen Anne's County, Maryland.