23 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Jackson | 1 | 3,217 | 3,321 | -0.53% | 620 | 5 | City |
| Lakefield | 2 | 1,698 | 1,734 | -0.35% | 1,269 | 1 | City |
| Jackson | 3 | 1,645 | 1,610 | 0.18% | 315 | 5 | Township |
| Heron Lake | 4 | 586 | 603 | -0.51% | 472 | 1 | City |
| Heron Lake | 5 | 298 | 309 | -1.32% | 8 | 39 | Township |
| Christiania | 6 | 264 | 267 | -0.38% | 7 | 36 | Township |
| Des Moines | 7 | 232 | 236 | -0.43% | 8 | 31 | Township |
| Delafield | 8 | 229 | 230 | 0% | 7 | 35 | Township |
| Petersburg | 9 | 217 | 221 | -0.46% | 6 | 36 | Township |
| Hunter | 10 | 215 | 216 | 0% | 6 | 36 | Township |
| Belmont | 11 | 214 | 217 | -0.47% | 6 | 36 | Township |
| Middletown | 12 | 213 | 214 | 0% | 6 | 36 | Township |
| Ewington | 13 | 207 | 208 | 0% | 6 | 36 | Township |
| Wisconsin | 14 | 207 | 210 | -0.48% | 6 | 35 | Township |
| Rost | 15 | 203 | 206 | -0.49% | 6 | 36 | Township |
| Okabena | 16 | 199 | 204 | -0.5% | 589 | 0 | City |
| Sioux Valley | 17 | 194 | 197 | -0.51% | 5 | 36 | Township |
| Enterprise | 18 | 179 | 182 | -0.56% | 5 | 36 | Township |
| Alba | 19 | 163 | 166 | -0.61% | 5 | 35 | Township |
| Weimer | 20 | 157 | 157 | 0% | 5 | 31 | Township |
| La Crosse | 21 | 146 | 146 | 0% | 4 | 35 | Township |
| West Heron Lake | 22 | 124 | 126 | -0.8% | 5 | 27 | Township |
| Alpha | 23 | 95 | 98 | 0% | 444 | 0 | City |
Jackson is the largest city in Jackson County, with a population of 3,217.
Alpha is the smallest city in Jackson County, with a population of 95.
There are 23 cities in Jackson County, Minnesota.