| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mondovi | 1 | 2,858 | 2,851 | 0.18% | 734 | 4 | City |
| Buffalo City | 2 | 1,009 | 1,011 | 0% | 533 | 2 | City |
| Fountain City | 3 | 762 | 803 | -0.91% | 174 | 4 | City |
| Alma | 4 | 720 | 718 | 0.28% | 136 | 5 | City |
| Cochrane | 5 | 415 | 426 | -0.24% | 536 | 1 | Village |
| Nelson | 6 | 314 | 320 | 0% | 232 | 1 | Village |
Mondovi is the largest city in Buffalo County, with a population of 2,858.
Nelson is the smallest city in Buffalo County, with a population of 314.
There are 6 cities in Buffalo County, Wisconsin.