| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mauston | 1 | 4,084 | 4,357 | -1.19% | 914 | 4 | City |
| New Lisbon | 2 | 2,579 | 2,575 | 0.08% | 944 | 3 | City |
| Elroy | 3 | 1,233 | 1,355 | -1.75% | 614 | 2 | City |
| Necedah | 4 | 906 | 936 | -0.55% | 328 | 3 | Village |
| Wonewoc | 5 | 715 | 759 | -1.11% | 640 | 1 | Village |
| Camp Douglas | 6 | 622 | 650 | -0.8% | 619 | 1 | Village |
| Lyndon Station | 7 | 469 | 494 | -0.85% | 222 | 2 | Village |
| Ironton | 8 | 257 | 273 | -1.15% | 772 | 0 | Village |
| Union Center | 9 | 218 | 229 | -0.91% | 265 | 1 | Village |
| Hustler | 10 | 157 | 166 | -1.26% | 225 | 1 | Village |
| Yuba | 11 | 50 | 52 | -1.96% | 164 | 0 | Village |
Mauston is the largest city in Juneau County, with a population of 4,084.
Yuba is the smallest city in Juneau County, with a population of 50.
There are 11 cities in Juneau County, Wisconsin.