3 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Sistersville | 1 | 1,275 | 1,405 | -2% | 2,429 | 0.53 | City |
| Middlebourne | 2 | 652 | 716 | -1.95% | 1,806 | 0.36 | Town |
| Friendly | 3 | 91 | 98 | -1.09% | 901 | 0.10 | Town |
Sistersville is the largest city in Tyler County, with a population of 1,275.
Friendly is the smallest city in Tyler County.
There are 3 cities in Tyler County, West Virginia.