45 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Calais | 1 | 3,095 | 3,084 | -0.16% | 90 | 34 | City |
| Machias | 2 | 2,028 | 2,063 | -0.78% | 146 | 14 | Town |
| Milbridge | 3 | 1,337 | 1,368 | -1.18% | 55 | 24 | Town |
| East Machias | 4 | 1,321 | 1,318 | -0.45% | 38 | 35 | Town |
| Baileyville | 5 | 1,297 | 1,319 | -0.54% | 35 | 37 | Town |
| Eastport | 6 | 1,284 | 1,283 | -0.31% | 353 | 4 | City |
| Jonesport | 7 | 1,237 | 1,240 | -0.56% | 43 | 29 | Town |
| Lubec | 8 | 1,229 | 1,234 | -0.32% | 37 | 33 | Town |
| Addison | 9 | 1,183 | 1,150 | 0.17% | 28 | 42 | Town |
| Steuben | 10 | 1,178 | 1,133 | 0.77% | 27 | 43 | Town |
| Cherryfield | 11 | 1,079 | 1,105 | -0.74% | 24 | 45 | Town |
| Machiasport | 12 | 1,013 | 973 | 0.1% | 47 | 21 | Town |
| Harrington | 13 | 998 | 965 | 0.6% | 47 | 21 | Town |
| Perry | 14 | 782 | 802 | -0.76% | 27 | 29 | Town |
| Pembroke | 15 | 777 | 787 | -0.38% | 28 | 27 | Town |
| Passamaquoddy Indian Township Reservation | 16 | 755 | 760 | -0.53% | 20 | 38 | Township |
| Princeton | 17 | 748 | 748 | -0.27% | 20 | 37 | Town |
| Passamaquoddy Pleasant Point Reservation | 18 | 689 | 690 | -0.14% | 1,219 | 1 | Township |
| Jonesboro | 19 | 610 | 578 | 0.16% | 17 | 37 | Town |
| Danforth | 20 | 590 | 589 | -0.17% | 11 | 54 | Town |
| Robbinston | 21 | 553 | 542 | 0% | 20 | 28 | Town |
| Marshfield | 22 | 543 | 529 | 0.74% | 32 | 17 | Town |
| Whiting | 23 | 525 | 487 | 2.34% | 11 | 47 | Town |
| Cutler | 24 | 522 | 522 | 0% | 11 | 47 | Town |
| Alexander | 25 | 514 | 523 | -0.58% | 13 | 40 | Town |
| Columbia Falls | 26 | 482 | 474 | -0.21% | 20 | 25 | Town |
| Beals | 27 | 460 | 446 | 0.44% | 82 | 6 | Town |
| Columbia | 28 | 434 | 434 | -0.23% | 12 | 36 | Town |
| Charlotte | 29 | 339 | 337 | 0% | 11 | 31 | Town |
| Roque Bluffs | 30 | 321 | 294 | 1.26% | 31 | 10 | Town |
| Dennysville | 31 | 314 | 302 | 0% | 21 | 15 | Town |
| Whitneyville | 32 | 204 | 202 | -0.49% | 14 | 15 | Town |
| Baring | 33 | 198 | 201 | -0.5% | 10 | 21 | Plantation |
| Topsfield | 34 | 178 | 180 | -0.56% | 4 | 50 | Town |
| Northfield | 35 | 176 | 178 | -0.56% | 4 | 44 | Town |
| Cooper | 36 | 169 | 172 | -0.59% | 6 | 31 | Town |
| Meddybemps | 37 | 136 | 136 | 0% | 10 | 13 | Town |
| Grand Lake Stream | 38 | 127 | 127 | 0% | 3 | 44 | Plantation |
| Wesley | 39 | 121 | 121 | 0% | 2 | 50 | Town |
| Vanceboro | 40 | 102 | 104 | -0.97% | 5 | 20 | Town |
| Crawford | 41 | 95 | 91 | 0% | 3 | 35 | Town |
| Deblois | 42 | 76 | 75 | 0% | 2 | 36 | Town |
| Talmadge | 43 | 70 | 70 | 0% | 2 | 38 | Town |
| Waite | 44 | 68 | 67 | 0% | 2 | 44 | Town |
| Beddington | 45 | 62 | 62 | 0% | 2 | 35 | Town |
Calais is the largest city in Washington County, with a population of 3,095.
Beddington is the smallest city in Washington County, with a population of 62.
There are 45 cities in Washington County, Maine.