26 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Parker | 1 | 1,333 | 1,198 | 2.07% | 1,137 | 1 | City |
| Centerville | 2 | 966 | 926 | 0.84% | 1,342 | 1 | City |
| Marion | 3 | 888 | 848 | 0.91% | 1,023 | 1 | City |
| Viborg | 4 | 840 | 808 | 0.72% | 1,618 | 1 | City |
| Home | 5 | 410 | 391 | 0.24% | 15 | 28 | Township |
| Hurley | 6 | 386 | 378 | 0.26% | 627 | 1 | City |
| Germantown | 7 | 357 | 344 | -0.28% | 10 | 36 | Township |
| Chancellor | 8 | 315 | 300 | 0.96% | 1,432 | 0 | Town |
| Parker | 9 | 279 | 263 | 0.72% | 8 | 35 | Township |
| Swan Lake | 10 | 268 | 257 | 0% | 8 | 35 | Township |
| Middleton | 11 | 262 | 252 | -0.38% | 7 | 35 | Township |
| Spring Valley | 12 | 242 | 235 | -0.41% | 7 | 36 | Township |
| Childstown | 13 | 213 | 205 | -0.47% | 6 | 36 | Township |
| Turner | 14 | 198 | 190 | -0.5% | 6 | 36 | Township |
| Daneville | 15 | 190 | 181 | 0% | 5 | 36 | Township |
| Marion | 16 | 190 | 179 | 1.06% | 5 | 35 | Township |
| Brothersfield | 17 | 186 | 173 | 1.64% | 7 | 28 | Township |
| Centerville | 18 | 186 | 178 | -0.53% | 5 | 35 | Township |
| Rosefield | 19 | 181 | 170 | 1.12% | 5 | 36 | Township |
| Monroe | 20 | 178 | 167 | 1.14% | 6 | 28 | Township |
| Hurley | 21 | 171 | 166 | -0.58% | 5 | 35 | Township |
| Norway | 22 | 166 | 157 | 0.61% | 5 | 36 | Township |
| Monroe | 23 | 164 | 159 | 0.61% | 433 | 0 | Town |
| Dolton | 24 | 120 | 115 | 0% | 4 | 29 | Township |
| Davis | 25 | 55 | 54 | 0% | 128 | 0 | Town |
| Centerville | 26 | 38 | 33 | 8.57% | 1 | 35 | Township |
Parker is the largest city in Turner County, with a population of 1,333.
Centerville is the smallest city in Turner County, with a population of 38.
There are 26 cities in Turner County, South Dakota.