14 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Tuscaloosa | 1 | 116,465 | 100,987 | 1.9% | 1,874 | 62 | City |
| Tuscaloosa | 2 | 116,465 | 105,578 | 1.9% | 1,874 | 62 | City |
| Northport | 3 | 31,563 | 31,198 | 0.23% | 1,767 | 18 | City |
| Northport | 4 | 31,563 | 31,208 | 0.23% | 1,767 | 18 | City |
| Cottondale | 5 | 4,014 | 3,325 | 7.15% | 1,166 | 3 | CDP |
| Holt | 6 | 3,672 | 4,358 | 0.05% | 1,161 | 3 | CDP |
| Brookwood | 7 | 2,441 | 2,503 | -0.49% | 292 | 8 | Town |
| Brookwood | 8 | 2,441 | 2,500 | -0.49% | 292 | 8 | Town |
| Vance | 9 | 2,344 | 2,100 | 1.78% | 230 | 10 | Town |
| Vance | 10 | 2,344 | 2,136 | 1.78% | 230 | 10 | Town |
| Coaling | 11 | 1,987 | 2,040 | -0.5% | 396 | 5 | Town |
| Coaling | 12 | 1,987 | 2,035 | -0.5% | 396 | 5 | Town |
| Coker | 13 | 885 | 904 | -0.45% | 454 | 2 | Town |
| Coker | 14 | 885 | 902 | -0.45% | 454 | 2 | Town |
Tuscaloosa is the largest city in Tuscaloosa County, with a population of 116,465.
Coker is the smallest city in Tuscaloosa County, with a population of 885.
There are 14 cities in Tuscaloosa County, Alabama.