| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Fort Madison | 1 | 9,861 | 10,310 | -0.74% | 1,022 | 10 | City |
| Keokuk | 2 | 9,417 | 9,885 | -0.98% | 1,035 | 9 | City |
| West Point | 3 | 896 | 927 | -0.55% | 1,409 | 1 | City |
| Donnellson | 4 | 789 | 890 | -2.35% | 620 | 1 | City |
| Montrose | 5 | 742 | 742 | -0.4% | 614 | 1 | City |
| Franklin | 6 | 134 | 131 | 0.75% | 859 | 0 | City |
| Houghton | 7 | 125 | 130 | -0.79% | 422 | 0 | City |
| St. Paul | 8 | 103 | 108 | -0.96% | 271 | 0 | City |
Fort Madison is the largest city in Lee County, with a population of 9,861.
St. Paul is the smallest city in Lee County, with a population of 103.
There are 8 cities in Lee County, Iowa.