4 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nocatee | 1 | 27,556 | 16,914 | 5.9% | 1,028 | 27 | CDP |
| Arcadia | 2 | 8,107 | 7,442 | 1.67% | 1,822 | 4 | City |
| Southeast Arcadia | 3 | 7,955 | 8,859 | 1.18% | 1,099 | 7 | CDP |
| Limestone | 4 | 7 | 320 | -53.33% | 0.00 | 24 | CDP |
Nocatee is the largest city in DeSoto County, with a population of 27,556.
Limestone is the smallest city in DeSoto County, with a population of 7.
There are 4 cities in DeSoto County, Florida.