4 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Lake City | 1 | 12,892 | 12,345 | 0.85% | 901 | 14 | City |
| Watertown | 2 | 2,614 | 2,381 | -0.53% | 1,057 | 2 | CDP |
| Five Points | 3 | 961 | 1,276 | -2.14% | 443 | 2 | CDP |
| Fort White | 4 | 681 | 621 | 1.79% | 266 | 3 | Town |
Lake City is the largest city in Columbia County, with a population of 12,892.
Fort White is the smallest city in Columbia County, with a population of 681.
There are 4 cities in Columbia County, Florida.