2 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Lake City | 1 | 13,002 | 12,345 | 0.85% | 899 | 14 | City |
| Fort White | 2 | 693 | 621 | 1.76% | 271 | 3 | Town |
Lake City is the largest city in Columbia County, with a population of 13,002.
Fort White is the smallest city in Columbia County, with a population of 693.
There are 2 cities in Columbia County, Florida.