4 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Lake City | 1 | 12,776 | 12,340 | 0.69% | 893 | 14 | City |
| Watertown | 2 | 2,614 | 2,381 | -0.53% | 1,057 | 2 | CDP |
| Five Points | 3 | 961 | 1,276 | -2.14% | 443 | 2 | CDP |
| Fort White | 4 | 667 | 619 | 1.52% | 261 | 3 | Town |
Lake City is the largest city in Columbia County, with a population of 12,776.
Fort White is the smallest city in Columbia County, with a population of 667.
There are 4 cities in Columbia County, Florida.