11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Gainesville | 1 | 148,190 | 142,245 | 0.81% | 2,316 | 64 | City |
| Alachua | 2 | 10,898 | 10,578 | 0.59% | 301 | 36 | City |
| Newberry | 3 | 8,413 | 7,405 | 2.46% | 139 | 60 | City |
| High Springs | 4 | 6,917 | 6,244 | 1.99% | 301 | 23 | City |
| East Bronson | 5 | 1,919 | 1,484 | -0.05% | 168 | 11 | CDP |
| Hawthorne | 6 | 1,513 | 1,487 | 0.33% | 205 | 7 | City |
| Archer | 7 | 1,161 | 1,143 | 0.35% | 127 | 9 | City |
| Waldo | 8 | 801 | 843 | -0.99% | 368 | 2 | City |
| Micanopy | 9 | 656 | 645 | 0.31% | 633 | 1 | Town |
| La Crosse | 10 | 323 | 315 | 0.62% | 70 | 5 | Town |
| Spring Ridge | 11 | 220 | 302 | -5.17% | 68 | 3 | CDP |
Gainesville is the largest city in Alachua County, with a population of 148,190.
Spring Ridge is the smallest city in Alachua County, with a population of 220.
There are 11 cities in Alachua County, Florida.